OpenPCR

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OpenPCR is a DIYBio project. OpenPCR is the name given to a kickstarter project that aimed to produce an open source version of a laboratory quality PCR machine and to significantly reduce the cost. We are using their designs as a template to build our own version for the DIYBio Groningen initiative, and hope to improve on the design in future iterations. The build is being done in cooperation with the FabLab using the tools and knowledge-base that exists there.

We wish to showcase the project with the Groningen hackerspace/makerspace and the FabLab at the Groningen mini-MakerFaire on October 12th.

Contents

Organisation

Organisation:

Workgroups:

Tasks

This task list is open to changes. The tasks are simple enough te be done by anyone. The lower tasks are building blocks for lower tasks. Some tasks can be done in parallel as we have access to three Arduino's.

Needed additionally: a thermometer

The first value is an estimate of the difficulty based an irrational scale. Tasks based in its results will have a higher difficulty estimate.

  • Wednesday, Claudio and Assert: 2/10: OpenPCR task 1: Calibrating thermistor on Arduino
  • DONE: 2/10: OpenPCR task 2: Connecting a relay to Arduino and find out the maximum switching frequency
  • DONE: 2/10: OpenPCR task 3: Connecting a Peltier element to a power supply
  • IMPOSSIBLE: 4/10: OpenPCR task 4 (after 2): Connecting an H bridge built from relays to an Arduino
  • 6/10: OpenPCR task 5 (after 3 and (14 or 15)): Let the Peltier element have its current switched by an H bridge
  • 1/10: OpenPCR task 6: Connecting a heating element to a power supply
  • 3/10: OpenPCR task 7 (after 2 and 6): Let the heating element have its current switched by a relay
  • 2/10: OpenPCR task 8: Redesign the heater element for smaller cups
  • 4/10: OpenPCR task 9: Let the heating element heat up the metal (after 7 and 8)
  • 7/10: OpenPCR task 10: Let the Peltier element (with H bridge) heat up the metal (after 5 and 8)
  • 8/10: OpenPCR task 11: (after 1-10) construct the machine its hardware by merging its parts
  • 8/10: OpenPCR task 12: (after 1-10) construct the machine its software by merging its parts
  • 9/10: OpenPCR task 13: (after 11) calibrate a PID control on the heating and cooling
  • 4/10: OpenPCR task 14 : Connecting an H bridge built from two NPN and two PNP transistors to an Arduino
  • 4/10: OpenPCR task 15 : Connecting an H bridge chip to an Arduino

Schedule

  • 2013-07-15: DIYBio meeting, (time?) @ Cafe de Minnaar
  • 2013-08-06: OpenPCR build session, 19:00 @ FabLab - Review BOM
  • 2013-08-08: OpenPCR build session, 17:30 @ Cafe de Minnaar - Ordering parts for Electronics
  • 2013-08-17: OpenPCR build session, 14:00 @ FabLab - review PCB, assemble electronics, setup Metal and Enclosure workgroups
  • 2013-08-20: OpenPCR build session, 19:00 @ FabLab - drill holes in PCB, solder SMD components
  • 2013-08-27: OpenPCR build session, 19:00 @ FabLab - drill holes in PCB, solder SMD components, re-order Kapton heater
  • 2013-08-28: OpenPCR build session, 11:00 @ University of Groningen - etching PCB
  • 2013-09-03: OpenPCR build session, 19:00 @ FabLab - drill holes in PCB, solder SMD components, order thermal pad and Mosfet
  • 2013-09-10: OpenPCR build session, 19:00 @ FabLab - complete PCB assembly
  • 2013-09-17: OpenPCR build session, 19:00 @ FabLab - complete PCB assembly, assemble metal parts, order/by screws and other mechanical parts for Enclosure
  • 2013-10-01: OpenPCR build session, 19:00 @ FabLab - metal parts, enclosure
  • 2013-10-08: OpenPCR build session, 19:00 @ FabLab
  • 2013-10-12: presentation and workshop @ Groningen mini-MakerFaire

Planned

  • 2013-10-19 and 2013-10-20: DIY Bio hacking weekend @ Assert

People involved

Add yourself here for eternal fame. No, we won't do it for you :)

Misc